Antimicrobial assay of the ethanol leaves extracts. Antimicrobial activities of moringa oleifera lam leaf extracts. Pdf antimicrobial activities of moringa oleifera lam leaf extracts. The leaves crude extract was fractionated into different fractions using solvents of.
Antimicrobial activity of methanol extract and fractions. Gram negative escherichia coli and acid fast mycobacterium phlei. Antimicrobial activity of different extracts of leaf of moringa oleifera. Then the extract was filtered with whatman 41 filter paper. Methanol extract 30 gdisc 30 gdisc 50 gdisc 100 gdisc staphylococcus aureus 30 mm 12 15 16 bacillus subtilis 29 mm 14 16. After that the paper disk which has been soaked in moringa oleifera l. Phytochemical screening and antimicrobial activities of ethanolic. The suspended bacterial test was inoculated on the surface of a sterile na medium using sterile swab. Moringa oleifera is a tropical tree whose many economic applications and facility of propagation are arousing growing international interest. However, not so extensive work on its antimicrobial properties has been. Original article phytochemical analysis and antifungal activity of moringa oleifera pinal patel 1, nivedita patel, dhara patel, sharav desai2, dhananjay meshram 1.
Department of microbiology, adekunle ajasin university, p. Dec 30, 2019 moringa oleifera is rich in various active phytoconstituents tannins, sterols, terpenoids, flavonoids, saponins, anthraquinones, alkaloids, and vitamins in addition to different minerals in its leaves and seeds. Dpph and frap methods were used to evaluate the antioxidant capacities of the extract fractions. Synthesis and characterization of zno nanoparticles using. Antioxidant activity and total phenolic content of moringa oleifera leaves in two stages of maturity. Evaluation of the biological activity of moringa oleifera. The medicinal value of moringa oleifera leaves and the antimicrobial activity of metallic copper were combined in the present study to synthesize copper nanoparticles having a desirable addedvalue inorganic material. Moringa oleifera was traditionally used in west sudan. Moringa oleifera is one of the vegetables of the brassica order and belongs to the family moringaceae.
Antibacterial activity of moringa oleifera and moringa. Accepted 3 february, 2014 moringa oleifera leaves extracted with methanol and dichloromethane were screened for antioxidant activity. Purification of small protein from moringa oleifera leaves. However, the water extract showed no activity at the highest concentration 5 mgml tested. Introduction moringa is the sole genus in the flowering plant family moringaceae and is native to asia and africa. Antioxidant, antimicrobial activity and mineral profile of. Moringaceae is a very useful tree in tropical countries. Moringaceae was tested for antimicrobial activities against gram positive bacillus cereus, bacillus subtilis staphylococcus aureus, sarcina lutea. Isolation, analysis and identification of phytochemicals of. Various parts of the plant such as the leaves, roots, seed, bark.
The moringa plant moringa oleifera has been the object of much research due to its multiple uses and wellknown bactericidal potential9,37,17,38. It is rich in nutrients and, apart from a range of industrial and medicinal applications, is used to purify water. Moringa oleifera is rich in various active phytoconstituents tannins, sterols, terpenoids, flavonoids, saponins, anthraquinones, alkaloids, and vitamins in addition to different minerals in its. Fraction p2 showed significant zone of inhibition against aspergillus niger. Hypoglycemic and antihyperglycemic activity of the. Antibacterial activity of leaf juice and extracts of moringa. The fresh leaf juice showed stronger antibacterial activity against studied gram negative bacteria and grampositive bacteria, the respective diameter zones. In addition, the moringa was found to have a group. Studies were conducted to determine the antimicrobial activities of moringa oleifera extracts using in vitro antimicrobial screening methods. Antioxidant and antimicrobial activity of drumstick moringa. Department of applied microbiology and brewing, nnamdi azikiwe university. The antimicrobial activities of moringa oleifera leaves, roots, bark and seeds were investigated in vitro against bacteria, yeast, dermatophytes and helminths pathogenic to man.
Extract was tested against two bacterial strains of gram positive bacteria bacillus subtilis. The ethnolic extract of the leaves of moringa oleifera lam. Preparation of moringa oleifera leaf extract 5 grams of moringa oleifera leaves were washed thoroughly with distilled water and both surface of leaves were sterilized using alcohol by gentle rubbing. Pdf antimicrobial activity of moringa oleifera lam. Antioxidant and anticancer activities of moringa oleifera leaves. Evaluation of antimicrobial properties and nutritional. Moringa oleifera leaf extracts was used for plant component analysis and for determination of antifungal activity. The paper disc diffusion method was used with treatments arranged in a completely randomized design and replicated four times. Molecules free fulltext green synthesis of encapsulated. Biofabrication of ag nanoparticles using moringa oleifera leaf extract and their antimicrobial activity tnvkv prasad 1, and ek elumalai 2 1 institute of frontier technology, regional agricultural research station, acharya n. Antimicrobal activity of moringa leaf extract the antimicrobial activity test of moringa oleifera l. Isolation, analysis and identification of phytochemicals. Root extract article pdf available in journal of pharmacy research. Antioxidant and anticancer activities of moringa oleifera.
Preparation of moringa oleifera leaf extract 5 grams of moringa oleifera leaves were washed thoroughly with distilled water and both surface of leaves were sterilized using alcohol by gentle. In the current study, in vitro antioxidant and antimicrobial activity assays of. Uses, side effects, interactions, dosage, and warning. The leaves crude extract was fractionated into different fractions using solvents of varying polarity. Preliminary screening for antimicrobial activity armando caceresa1, ofyluz cabrera1, ofelia morales1, patricia mollmedo and patricia mendia1 centeror mesownericun studies on appropriate technology oem at. In the current study, in vitro antioxidant and antimicrobial activity assays of aqueous, methanolic and ethanolic extracts from four different parts of m. Various parts of the plant such as the leaves, roots, seed, bark, fruit, possess antitumor. Accepted 3 february, 2014 moringa oleifera leaves extracted with methanol and dichloromethane were screened. The present study was carried out to determine the antimicrobial activity of aqueous and methanol. Purification steps volume ml total protein mg % yield crude 100. Antibacterial activity of moringa oleifera extracts on bacterial isolates showed that moringa oleifera leaf ethanol mle extract had the broadest spectrum of activity on the test bacteria.
The antibacterial activity of methanol and nhexane extracts of moringa oleifera andmoringa stenopetala seeds was conducted on 3 bacterial species salmonella typhii, vibrio cholerae and escherichia coli. The concentrated extract was stored at 4c until further use 15 antimicrobial assay of the ethanolic leaf extracts. Antimicrobial activity of moringa oleifera leaf against. Antimicrobial activities of moringa oleifera lam leaf. Antimicrobial activity of methanol extract and fractions of moringa oleifera lam. Antimicrobial activity and phytochemical analysis of moringa. View of antibacterial activity of moringa oleifera lam. The result exhibited that mle had antimicrobial activity against five bacterial isolates. Biofabrication of ag nanoparticles using moringa oleifera. Furthermore, moringa oleifera extracts suggested by many recent investigations as resistancemodifying agents against a wide array of pathogens 8,1215. Antioxidant and antimicrobial activity of drumstick. Phytochemical and antibacterial activity of moringa. Phytochemical screening, nutritional composition and. Comparison of the antimicrobial activities of the leaves crude extracts of moringa peregrina and moringa oleifera in saudi arabia mohamed abdelhamid elawady1,2, mohamed mahmoud hassan1,3, elsayed saleh abdelhameed4,5 and ahmed gaber1,2 1scientific research center, biotechnology and genetic engineering unit, taif university.
Antibacterial effect of the ethanol leaves extract of. Evaluation of the antimicrobial activity of moringa oleifera leaves extract on helicobacter pylori 1ezugwu, r. Department of applied microbiology and brewing, nnamdi azikiwe. Silver nanoparticle formation was confirmed by surface plasmon resonance at 450 nm and 440 nm, respectively for both fresh and freezedried. Nutritional quality of moringa oleifera for its bioactivity. Pdf antimicrobial activity of moringa oleifera from different.
Antibacterial effect in vitro of moringa oleifera and. Paper open access antimicrobial activity of moringa leaf. This study was designed to evaluate the antimicrobial potentials of ethanol and aqueous extracts of moringa oleifera against microorganisms isolated from beef offal. In this study we report on the synthesis of silver nanoparticles agnps from the leaf extracts of moringa oleifera using sunlight irradiation as primary source of energy, and its antimicrobial potential. In vitro antimicrobial activity of the fruit extract of moringa oleifera name of the bacteria zone of the inhibition in mm kanamycin disc standard moringa oleifera lam. The antibacterial evaluation of moringa oleifera leaf. Antimicrobial activity of different extracts of leaf of moringa oleifera lam against gram positive and gram negative bacteria. The antimicrobial study of leaves of moringa oleifera was evaluated for potential. Moringa oleifera, phytochemicals, nutrient content, antimicrobial activity, gastrointestinal pathogens i. Evaluation of antimicrobial properties and nutritional potentials of.
Biofabrication of ag nanoparticles using moringa oleifera leaf extract and their antimicrobial activity tnvkv prasad 1, and ek elumalai 2 1 institute of frontier technology, regional agricultural. Antimicrobial activity and phytochemical analysis of. This study was shaped to discover the antimicrobial, antioxidant and mineral profile of moringa oleifera leaves extracts. Phytochemical and antibacterial activity of moringa oleifera. The moringa oleifera plant samples like moringa leaf 1 mol1, moringa leaf 2 mol2, moringa seed mos and moringa flower mof were collected from the fields and taken to department for biotechnology, karunya university, coimbatore for identification and evaluation of antimicrobial activity of the plant sample. The medicinal value of moringa oleifera leaves and the antimicrobial activity of metallic copper were. The full text of this article is available as a pdf 32k. Green synthesis of silver nanoparticles from moringa. Aug 22, 1994 the ethnolic extract of the leaves of moringa oleifera lam. Studies were conducted to determine the antimicrobial activities of moringa oleifera extracts using in vitro. Effect of methanol extract of moringa oleifera leaves on. In vitro antimicrobial activity of methanolic extract of.
Antimicrobial activity of moringa oleifera hydroalcoholic. This study was conducted to investigate pragmatic extraction techniques for seed and leaf extracts of m. Antioxidative and antimicrobial activities of different. These leaves were heated for 40 min in 100 ml of distilled water at 50o c. The result reveals the presence of alkaloids, flavonoids and saponins in the chloroform and aqueous extracts of. The synthesis of metal nanoparticles using plant extracts is a very promising method in green synthesis. Leaves of moringa plant were collected in a clean bag within the town of akungbaakoko, ondo state, nigeria.
Antibacterial effect of the ethanol leaves extract of moringa. Root bark on clinical isolates of methicillin resistant staphylococcus aureus with abstract, chapter 15, references and questionnaire. Comparison of the antimicrobial activities of the leavescrude extracts of moringa peregrina and moringa oleifera in saudi arabia mohamed abdelhamid elawady1,2, mohamed mahmoud hassan1,3. The moringaceae is a single genus family with known species khawaja et al. Plants have been reported to contain important preventative and curative compounds. Antioxidant and anticancer activities of moringa oleifera leaves suphachai charoensin division of biochemistry and nutrition, school of medical sciences, university of phayao, phaholyothin rd. Introduction moringa oleifera is one of the most widely cultivated species of monogenic. Drumstick moringa oleifera leaves were evaluated for antioxidative capacity and antimicrobial activity when incorporated in chicken sausages. Significant antimicrobial activity of the extract was found in this study. Moringa leaf, antimicrobial activity, bacteria, fungi. The plant leaves are very good nutrient supplement for malnutrition and also used as an antibiotic.
Green synthesis of silver nanoparticles from moringa oleifera. The present study was designed to investigate the interactions between the methnol extract of moringa oleifera leaves mml and. Moringa oleifera leaves suphachai charoensin division of biochemistry and nutrition, school of medical sciences, university of phayao, phaholyothin rd. Moringaceae was tested for antimicrobial activities against gram positive bacillus cereus, bacillus subtilis. Original article antimicrobial activities and phytochemical analysis of moringa oleifera leaves on staphylococus aureus and streptococcus species ajayi a.
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